Prolonged Hormone Secretion from Neuroendocrine Cells of Aplysia is Indepentdent of Extracellular Calcium
Identifieur interne : 000809 ( Main/Exploration ); précédent : 000808; suivant : 000810Prolonged Hormone Secretion from Neuroendocrine Cells of Aplysia is Indepentdent of Extracellular Calcium
Auteurs : Nancy L. Wayne [États-Unis] ; Jiin Kim [États-Unis] ; Eugene Lee [États-Unis]Source :
- Journal of Neuroendocrinology [ 0953-8194 ] ; 1998-07.
English descriptors
- Teeft :
- Abdominal ganglion, Acad, Action potentials, Afterdischarge, Afterdischarges, Aplysia, Aplysia californica, Bapta, Baseline, Blackwell science, Calcium, Calcium channels, Calcium ionophore, Calcium ionophores, Californica, Cell afterdischarge, Cell neurons, Chelators, Comp physiol, Control group, Control preparations, Depolarization, Egta, Electrical activity, Electrical afterdischarge, Electrical stimulation, Exocytosis, Experimental group, Extracellular, Extracellular calcium, Extracellular calcium chelators, Ganglion, Hormone secretion, Intracellular, Intracellular stores, Ionophore, Kaczmarek, Kinase, Natl, Ndings, Neuroendocrine, Neuroendocrine cells, Neuroendocrinology, Neuron, Neurosecretion, Peptide, Physiol, Present study, Pretreatment period, Proc, Proc natl acad, Protein kinase, Retinal neuron, Secretion, Secretory, Total amount, Treatment period, Vascular space.
Abstract
The role of Ca2+ from extracellular and intracellular sources in stimulating neurosecretion was investigated in four experiments using neuroendocrine bag cells of the marine mollusk Aplysia. (i) Bag cells were treated with either an extracellular calcium chelator (BAPTA) or Co2+‐substitution within 30 s after onset of an electrical afterdischarge to prevent influx of Ca2+ from extracellular fluid. These treatments shortened the duration of the afterdischarge, but did not significantly affect the overall pattern or total amount of egg laying hormone (ELH) secretion, suggesting that extracellular Ca2+ is not required for maintenance of ELH release. (ii) Substitution of Ba2+ for Ca2+ has previously been shown to support bag cell afterdischarges that trigger transient elevations in intracellular Ca2+. We showed that this treatment also stimulates ELH secretion, suggesting that Ca2+ release from intracellular stores can stimulate ELH secretion. (iii) To raise intracellular Ca2+ levels in the absence of an afterdischarge, the calcium ionophore X537A was used to transport Ca2+ across plasma and organelle membranes. When this treatment was combined with extracellular calcium chelators so that the only source of Ca2+ was from intracellular compartments, ELH secretion was stimulated. Taken together, these findings are consistent with the hypothesis that release of Ca2+ from intracellular stores is sufficient to stimulate ELH secretion.
Url:
DOI: 10.1046/j.1365-2826.1998.00235.x
Affiliations:
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<publisher>Blackwell Science Ltd, UK</publisher>
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<term>Action potentials</term>
<term>Afterdischarge</term>
<term>Afterdischarges</term>
<term>Aplysia</term>
<term>Aplysia californica</term>
<term>Bapta</term>
<term>Baseline</term>
<term>Blackwell science</term>
<term>Calcium</term>
<term>Calcium channels</term>
<term>Calcium ionophore</term>
<term>Calcium ionophores</term>
<term>Californica</term>
<term>Cell afterdischarge</term>
<term>Cell neurons</term>
<term>Chelators</term>
<term>Comp physiol</term>
<term>Control group</term>
<term>Control preparations</term>
<term>Depolarization</term>
<term>Egta</term>
<term>Electrical activity</term>
<term>Electrical afterdischarge</term>
<term>Electrical stimulation</term>
<term>Exocytosis</term>
<term>Experimental group</term>
<term>Extracellular</term>
<term>Extracellular calcium</term>
<term>Extracellular calcium chelators</term>
<term>Ganglion</term>
<term>Hormone secretion</term>
<term>Intracellular</term>
<term>Intracellular stores</term>
<term>Ionophore</term>
<term>Kaczmarek</term>
<term>Kinase</term>
<term>Natl</term>
<term>Ndings</term>
<term>Neuroendocrine</term>
<term>Neuroendocrine cells</term>
<term>Neuroendocrinology</term>
<term>Neuron</term>
<term>Neurosecretion</term>
<term>Peptide</term>
<term>Physiol</term>
<term>Present study</term>
<term>Pretreatment period</term>
<term>Proc</term>
<term>Proc natl acad</term>
<term>Protein kinase</term>
<term>Retinal neuron</term>
<term>Secretion</term>
<term>Secretory</term>
<term>Total amount</term>
<term>Treatment period</term>
<term>Vascular space</term>
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<front><div type="abstract" xml:lang="en">The role of Ca2+ from extracellular and intracellular sources in stimulating neurosecretion was investigated in four experiments using neuroendocrine bag cells of the marine mollusk Aplysia. (i) Bag cells were treated with either an extracellular calcium chelator (BAPTA) or Co2+‐substitution within 30 s after onset of an electrical afterdischarge to prevent influx of Ca2+ from extracellular fluid. These treatments shortened the duration of the afterdischarge, but did not significantly affect the overall pattern or total amount of egg laying hormone (ELH) secretion, suggesting that extracellular Ca2+ is not required for maintenance of ELH release. (ii) Substitution of Ba2+ for Ca2+ has previously been shown to support bag cell afterdischarges that trigger transient elevations in intracellular Ca2+. We showed that this treatment also stimulates ELH secretion, suggesting that Ca2+ release from intracellular stores can stimulate ELH secretion. (iii) To raise intracellular Ca2+ levels in the absence of an afterdischarge, the calcium ionophore X537A was used to transport Ca2+ across plasma and organelle membranes. When this treatment was combined with extracellular calcium chelators so that the only source of Ca2+ was from intracellular compartments, ELH secretion was stimulated. Taken together, these findings are consistent with the hypothesis that release of Ca2+ from intracellular stores is sufficient to stimulate ELH secretion.</div>
</front>
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